7 aad Search Results


amino actinomycin d 7 aad apoptosis detection kit  (Sino Biological)
93
Sino Biological amino actinomycin d 7 aad apoptosis detection kit
Amino Actinomycin D 7 Aad Apoptosis Detection Kit, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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7aad  (Miltenyi Biotec)
99
Miltenyi Biotec 7aad
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
7aad, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v pe  (Vazyme Biotech Co)
96
Vazyme Biotech Co annexin v pe
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
Annexin V Pe, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v apc  (Multi Sciences (Lianke) Biotech Co Ltd)
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Multi Sciences (Lianke) Biotech Co Ltd annexin v apc
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
Annexin V Apc, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v pe  (Multi Sciences (Lianke) Biotech Co Ltd)
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Multi Sciences (Lianke) Biotech Co Ltd annexin v pe
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
Annexin V Pe, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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7 aad reagent  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc 7 aad reagent
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
7 Aad Reagent, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v fitc apoptosis detection kit  (Cytek Biosciences)
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Cytek Biosciences annexin v fitc apoptosis detection kit
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
Annexin V Fitc Apoptosis Detection Kit, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aminoactinomycin d 7 aad  (Biotium)
93
Biotium aminoactinomycin d 7 aad
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
Aminoactinomycin D 7 Aad, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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7 aminoactinomycin d  (MedChemExpress)
94
MedChemExpress 7 aminoactinomycin d
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
7 Aminoactinomycin D, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aad cell staining solution  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc aad cell staining solution
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
Aad Cell Staining Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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7 aad kit  (Multi Sciences (Lianke) Biotech Co Ltd)
95
Multi Sciences (Lianke) Biotech Co Ltd 7 aad kit
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
7 Aad Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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readidrop 7 aad cell viability dye  (Bio-Rad)
93
Bio-Rad readidrop 7 aad cell viability dye
Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with <t>7AAD</t> dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.
Readidrop 7 Aad Cell Viability Dye, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with 7AAD dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.

Journal: Iranian journal of allergy, asthma, and immunology

Article Title: Construction and Functional Characterization of a Fully Human Anti-mesothelin Chimeric Antigen Receptor (CAR) Expressing T Cell.

doi: 10.18502/ijaai.v19i3.3454

Figure Lengend Snippet: Figure 3. Strong cytotoxic activity of MSLN-CAR T cells. MSLN-CAR T, Un-T, and Mock cells were co-cultured at 1:1, 5:1, 10:1, and 20:1 ratios with PKH26 labeled Hela, OVCAR3, and NALM-6 cells for 18 hours and then stained with 7AAD dye. the cytotoxic function of CAR T cells was evaluated by comparison of the percentage of lysed target cells (PKH26+/7AAD+ cells) with the negative control (cultured PKH26 labeled target cells without effector T cells). A) Representative flowcytometry histograms show cytotoxicity of MSLN-CAR T cells against NALM-6, Hela, and OVCAR3 cells and Mock and Un-T cells against Hela cells at an effector target ratio 10:1. B) Line plots-display cytotoxicity of MSLN-CAR T cells against Hela, OVCAR3, and NALM-6, also Un-T and Mock cells against Hela cells. Statistical analysis was performed by one-way ANOVA following Tukey’s post hoc. Data are representative of six independent experiments and presented as mean±SD. p<0.05 was considered statistically significant. 7AAD: 7-Aminoactinomycin D. Un-T: un-transduced T cell.

Article Snippet: 7AAD (Milteny Biotech, Germany) was used as a viability dye for flow cytometry.

Techniques: Activity Assay, Cell Culture, Labeling, Staining, Comparison, Negative Control